Coding

Part:BBa_K1885120:Design

Designed by: Kien Patrick Malarney   Group: iGEM16_ASIJ_Tokyo   (2016-10-29)


PETase from Ideonella Sakainesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 864
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 864
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 688
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 864
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 864
    Illegal NgoMIV site found at 139
    Illegal NgoMIV site found at 193
    Illegal NgoMIV site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was taken directly from the genome sequence.


Source

This part comes from a shotgun sequence of the Ideonella Sakainesis genome.

References